BioScience Trends. 2017;11(4):181-185. (DOI: 10.5582/ddt.2017.01029)

Nanoemulsion: A suitable nanodelivery system of clove oil for anesthetizing Nile tilapia.

Kheawfu K, Pikulkaew S, Chaisri W, Okonogi S


SUMMARY

Clove oil ethanolic solution (CL-EtOH) have always been used for fish anesthesia. However, ethanol causes major side effect of fish hypersensivity. In this study, clove oil loaded nanoemulsion (CLN) was developed in order to enhance water miscibility of clove oil without using ethanol in the preparations. The obtained CLN was characterized in terms of droplet size, size distribution expressed as polydispersity index (PDI), and zeta potential. The anesthetic effect of CLN in comparison with CL-EtOH on Oreochromis niloticus (Nile tilapia) was investigated. The results showed that the best CLN was composed of 20% w/w clove oil and 15% w/w polysorbate 20. This CLN has internal droplet size of 63.2


KEYWORDS: Nanoemulsion, clove oil, fish anesthesia, Nile tilapia, Oreochromis niloticus

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