BioScience Trends. 2018;12(2):88-91. (DOI: 10.5582/ddt.2018.01019)

Estimation of lactic acid bacterial cell number by DNA quantification.

Ishii M, Matsumoto Y, Sekimizu K


SUMMARY

Lactic acid bacteria are provided by fermented foods, beverages, medicines, and supplements. Because the beneficial effects of medicines and supplements containing functional lactic acid bacteria are related to the bacterial cell number, it is important to establish a simple method for estimating the total number of lactic acid bacterial cells in the products for quality control. Almost all of the lactic acid bacteria in the products are dead, however, making it difficult to estimate the total number of lactic acid bacterial cells in the products using a standard colonycounting method. Here we estimated the total lactic acid bacterial cell number in samples containing dead bacteria by quantifying the DNA. The number of viable Enterococcus faecalis 0831-07 cells decreased to less than 1


KEYWORDS: Lactic acid bacteria, medicines, supplements, DNA quantification, total cell number estimation

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