BioScience Trends. 2015;9(2):123-128. (DOI: 10.5582/ddt.2015.01024)
Galactosylation of caffeic acid by an engineered ?-galactosidase.
Lu LL, Guo YC, Xu LJ, Qi TT, Jin L, Xu L, Xiao M
Glycosylation is useful for improving the chemical properties and physiological functions of biologically and pharmacologically important compounds. The glycosylation of phenolic compounds can increase their solubility and stability in water. The addition of galactose residue has special meaning as it facilitates targeted delivery of drugs to the liver cancer cells with abundant galactose acceptors on the cell surface. In this work, the engineered ?-galactosidase W980F from Lactobacillus bulgaricus L3 was utilized for the glycosylation of caffeic acid, a well-known phenolic phytochemical with broad bioactivities. The reaction was performed by incubation of the enzyme with 200 mM of lactose and 100 mM of caffeic acid at 45
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